Tamer Abu Ghazaleh was born in Cairo to a family of Palestinian exiles. He was exposed to music at an early age and began composing at a young age. In 1998, he returned to Palestine and began his studies in music theory and composition at the National Conservatory in Ramallah (now the Edward Said Conservatory), while also deepening his skills on the oud and buzuq.

In 1991, under the influence of the First Intifada, he released his first single Ma Fi Khof ("There is no fear"). In 2001, his debut album Janayen El Ghona ("Gardens of Song") was released, containing compositions from his early years (between the ages of 5 and 15). In 2008, Mir'ah ("Mirror") was released, and in 2016, Thulth, his third album, according to the Arabic word meaning.

In 2007, he founded the organization eka3 as an "incubator for the Arab music industry". This has resulted in a record label (Mostakell), a booking agency (Almoharek) and a licensing agency (Awyav). He also helped found the online music magazine Ma3azef.com. 

In 2012, the Cairo-based alternative band Alif was formed, consisting of Abu Ghazaleh, Maurice Louca and Khyam Allami. Abu Ghazaleh is also a member of the 2010 crossover band Kazamada with Zeid Hamdan, Mahmoud Radaideh and Donia Massoud. He also worked as a producer and performed in numerous collaborations with Egyptian and Palestinian artists. He has performed with his projects in various cities in the Arab world and Europe.

Abu Ghazaleh sings in the idiom of his homeland, the Palestinian Arabic dialect. He uses satire and mockery to comment on the political and social issues of the present, and uses literary texts from the past in his music. Abu Ghazaleh combines the rich musical traditions of the Arab world with contemporary world music and jazz influences, and incorporates electronic sounds into his sound, for example in the style of Maghrebian electro-chaabi. His third album Thulth is characterized by many dynamic changes; the music magazine Melody and Rhythm emphasized the "surprising breaks" and the experimental character of the album. Similarly, the magazine jazzthetics underlines its great "intensity" and richness of contrasts.